Template-Type: ReDIF-Article 1.0 Author-Name:Muhammad Zia Ullah, Beenish Rasheed, Adnan Akhter, Waheed Akram, Muhammad Abu Bakar Siddique, Muhammad Khurshid, Hafiz Muhammad Tariq, Nasir Ali, Umair Raza, Muhammad Ahmad Author-Email:adnanakhter.iags@pu.edu.pk Author-Workplace-Name:Department of Plant Pathology, University of the Punjab Lahore, Pakistan, School of Biochemistry and Biotechnology, University of the Punjab Lahore, Pakistan, Department of Plant Pathology, Bahauddin Zakariya University Multan, Pakistan Title:Synergistic Effectof PyrolyzedBagasseand Trichoderma Viridefor Sustainable Mitigation of Chili Southern Blight Abstract:Soil-borne diseases like southern blight severely limit chili (Capsicum annuumL.) production, demanding sustainable and eco-friendly management approaches. This study introduces the integration of sugarcane bagasse-derived biochar with Trichoderma virideas a novel strategy for enhancing chili resistance against Sclerotium rolfsii. Biochar was produced through pyrolysis at 450°C and characterized using SEM, EDX, and XRD, revealing porous honeycomb-like structures, high carbon content, and mineral phases such as SiO₂and CaO. Glasshouse experiments were conducted on the chili cultivar ‘Desi’using biochar at 3%& 6% (v/v) concentrations. Biochar was either applied alone or in combination with T. virideas well as with S. rolfsii. Results demonstrated that biochar treatments significantly enhanced shoot and root growth, biomass accumulation, and physiological performance under pathogen stress. Disease severity, incidence, and mortality were notably reduced, with the greatest suppression (20%) noted in chili plants treated with 6% biochar plus T. viride. Furthermore, higher biochar doses substantially elevated levels of defense-related compounds, including phenolics, catalase, and flavonoids, indicating induction of systemic resistance. Similarly, the combined effect of biochar and T. viridewas also visible under in vitro assays. Overall, the integration of biochar and beneficial fungi not only improved soil health but also strengthened host defense, offering a sustainable approach to managingsouthern blight. These findings highlight biochar-induced resistance as a promising component of integrated disease management in chili cultivation. Keywords:Organic Amendments, Plant Protection, SclerotiumRolfsen,Biological Control, XRD Journal: International Journal of Innovations in Science and Technology Pages:2197-2212 Volume:7 Issue:3 Year: 2025 Month:September File-URL:https://journal.50sea.com/index.php/IJIST/article/view/1562/2196 File-Format: Application/pdf File-URL:https://journal.50sea.com/index.php/IJIST/article/view/1562 File-Format: text/html Handle: RePEc:abq:IJIST:v:7:y:2025:i:3:p:2197-2212